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KMID : 1189420180310040001
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2018 Volume.31 No. 4 p.1 ~ p.12
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The anti-oxidant and anti-inflammatory Activites of Changchulgeumryeontang Extract
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Won Hea-Ryeon
Park Hye-Su
Kim Ee-Hwa
Kim Yong-Min
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Abstract
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Objectives : This study investigated the anti-oxidant and anti-inflammatory activities of Changchulgeumryeontang (CCGRT) extract.
Methods : The macrophage cell line RAW 264.7 cells were used and MTT assay was performed to measure the cell viabilities at the various concentrations of CCGRT (25-200㎍/㎖). Nitric oxide (NO) and prostaglandin E2 (PGE2) were measured in LPS-induced RAW 264.7 cells. Expressions of iNOS, NF-κB, IL-1α, IL-1β and IL-6 were also performed by real-time PCR. The anti-oxidant activities of CCGRT was measured by DPPH radical scavenging activity.
Results : 1. there was no cytotoxicity in RAW264.7 cells treated with CCGRT compared to the control. 2. CCGRT treated group significantly inhibited NO and PGE 2 production compared to the LPS treated group. 3. CCGRT treated group significantly decreased mRNA expressions of iNOS, NF-κB, IL-1α, IL-1β and IL-6 compared to the LPS treated group. 4. CCGTR was found to have high DPPH free radical scavenging ability.
Conclusions : According to the above results, CCGRT may be a potentional choice for the treatment of inflammatory skin disease.
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KEYWORD
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Anti-inflammation , Anti-oxidant , nitric oxide (NO) , Prostaglandin E2 (PGE2) , Changchulgeumreontang (CCGRT)
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